PE-22-28: A Laboratory Research Reference

PE-22-28 is a synthetic heptapeptide derived from the peptide spadin. It is studied in laboratory research as a selective antagonist of the two-pore potassium channel TREK-1 in experimental models.

This page is a non-promotional scientific reference for researchers. It summarises the chemical identity, molecular characteristics, mechanism as described in the literature, research areas, and analytical testing of PE-22-28. It does not provide dosing, therapeutic, or human-use guidance of any kind. PE-22-28 supplied by Bulk Aussie Peptides is for laboratory research purposes only.

What Is PE-22-28?

PE-22-28 is a seven-amino-acid peptide engineered from spadin, a peptide derived from the sortilin precursor that was identified in studies of mood regulation and stress response. PE-22-28 spans residues 22 through 28 of the spadin sequence and functions as a shortened lead compound with improved potency and plasma stability relative to the parent peptide.

For background on how synthetic research peptides are produced and verified, see the synthetic peptides research guide.

Molecular Characteristics

• Classification: a synthetic heptapeptide derived from the sortilin-derived peptide spadin.
• Sequence: a seven-amino-acid fragment corresponding to spadin residues 22–28.
• Molecular weight: reported in Daltons (Da) on product documentation; the measured value on a Certificate of Analysis should be compared against the theoretical mass.
• Physical form: supplied as a lyophilised (freeze-dried) powder, the standard presentation for research peptides.

How PE-22-28 Works

PE-22-28 acts as a selective antagonist of TREK-1, a two-pore-domain potassium channel involved in setting the resting membrane potential and controlling neuronal excitability. In laboratory models, blockade of TREK-1 by PE-22-28 is associated with effects on neuronal membrane properties, hippocampal neurogenesis, and synaptic plasticity. The compound is investigated for its rapid-onset effects in depression-related behavioural models, though all findings remain preclinical.

These observations describe ion-channel pharmacology in model systems and do not constitute a therapeutic use.

Areas of Scientific Research

• TREK-1 channel pharmacology: characterisation of channel blockade and selectivity.
• Neuronal excitability: studies of membrane-potential and synaptic changes in neuronal models.
• Structure–activity relationships: work dissecting which spadin residues contribute to TREK-1 activity.

These investigations are conducted in vitro or in approved animal research models under institutional oversight. They do not translate to approved human use.

Analytical Testing

• HPLC for purity assessment, reported as a percentage of total peak area.
• Mass spectrometry for identity confirmation against the theoretical molecular mass.

Guidance on reading the resulting documents is available in the article on how to read a peptide Certificate of Analysis.

Storage and Handling

In a laboratory context, lyophilised PE-22-28 is typically stored in a freezer, protected from light and moisture, and handled according to the product-specific documentation. General guidance is covered in the peptide storage and stability reference.

Frequently Asked Questions

• What is PE-22-28? PE-22-28 is a synthetic heptapeptide derived from the peptide spadin.
• What does PE-22-28 target? It acts as a selective antagonist of the TREK-1 potassium channel.
• What is spadin? Spadin is a sortilin-derived peptide identified in studies of mood regulation.
• What is PE-22-28 studied for? It is investigated in laboratory models for TREK-1 pharmacology and neuronal excitability.
• How is PE-22-28 purity tested? By HPLC, with identity confirmed by mass spectrometry.

References

• Mazella, J., Pétrault, O., Lucas, G., Deval, E., Béraud-Dufour, S., Gandin, C., et al. (2010). Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: A new concept in the antidepressant drug design. PLOS Biology, 8(4), e1000355. PubMed 20405001

Related Research Resources

• Synthetic Peptides: Research Guide — how research peptides are produced and verified.
• Reading a Peptide Certificate of Analysis — interpreting purity and identity data.